The Genome in a Box project is the brainchild of researchers Anthony Birnie and Cees Dekker from the Dept. of Bionanoscience at the Delft University of Technology. Their stated goal is to assemble a functioning chromosome from the bottom-up, beginning with the naked DNA. In theory, the raw sequence could be printed in pieces using DNA synthesis machines and then stitched together into one long string with the correct code of the desired chromosome. That would be nearly impossible in practice, at least with our existing technology. There would be no way to keep the fragile strings sorted so that they could be properly joined and folded without error.
source https://phys.org/news/2021-01-d-creature-chromosomes-ground.html
